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mRNA relative expression was determined by 2 −ΔΔCt , with β-actin used as an internal reference gene

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Thus, model fit was assessed using the Comparative Fit Index, Root Mean Square Error of Approximation and Standardized Root Mean Square Residual as calculated by lavaan ‘cfa’ function, and Gamma Hat as calculated by the “moreFitIndices” function from semTools (v0.5–2) in RStudio (v3.5.1)